12/29/2023 0 Comments Logger pro absorbance![]() To calibrate the device, choose Calibrate > Spectrometer from the Experiment menu.To use the spectrometer in absorbance mode the spectrometer must be calibrated:.Wavelength will be displayed of the visible spectrum in nanometers superimposed over the colors associated with those wavelengths. The software will automatically identify the spectrometer.Device drivers were included with the Logger Pro 3 software. Note: Do not go online for device drivers.The first time you connect a SpectroVis Plus, your computer may ask you a few questions. Connect the spectrometer to the computer via a USB port.Requires Logger Pro 3 (Version 3.8.2 or higher) when using with a computer. Go to for the complete instructions or see the hard copy in the prep room. These instructions are taken from the Specifications sheet provided by Vernier. The OD ses of the undiluted protein is 1.673 The ODof a 1:10 dilution of your protein is 0.875 I First decide wliides of these two data points to use (1.673 or 0.Vernier Spectro-Vis Plus Spectrophotometer Overview The first, is an undiluted protein sample the second is a 1:20 dilution, made by adding 100u of original Nettie protein to 9001 of visulled water. 0.4 Regression Equation: y = 0.9429x + 0.1196 8 : 0 0.5 2 Concentration (mg/ml) You make two versions of the Nettie protein to measure via the Bradford Assuy. In a Bradford Assay, you generate the following standard curve and regression equation: Ooms 12 2. You are trying to determine the concentration of the protein Nettle that you have isolated by ammonium surate predpitation followed by size exclusion chromatography. Absorbance 1.4 1.2+ Maximum Absorbance Graph plateaus due to all the dye being bound by protein Absorbance A (00) 0.8 0.61 Maximum protein concentration 0.4 0.2 10 15 Concentration / Absorbance 1.4 12 Maximum Absorbance Graph plateaus due to all the dye being bound by protein sorbance A 100 0.8 Maximurġ.0 Bradford Assay Standard Curve Protein concentration v. This concept is illustrated by the graph below: 16 Bradford Assay Standard Curve Protein concentration v. That is, you can increase the protein level to a point where available dye becomes exhausted so the graph would plateau. As the dye is limiting (i.e., you only put in a set amount), then you can reach a point when all the dye is bound, and no а matter how much more protein you put in you will not see a change in absorbance. That is, the Bradford assay is really measuring the amount of bound dye and NOT the amount of protein present (although the two are connected until the dye is all used). The Bradford assay is based on a shift in the absorbance of the dye, Coomassie Brilliant Blue G-250, from 465 nm to 595 nm when it binds a protein under acidic conditions. The assay is an indirect (or colorimetric) method for estimating protein concentrations. The Bradford assay has limits, it does not work for all protein concentrations. In which term (% transmission or absorbance) are the data most usefully expressed? Why do we tend to use one set of values and not the other? (Hint: it's a math thing) 1 ![]() What is the difference between % transmission and absorbance? 6. Spectrophotometers can measure either %transmission or absorbance. howhich wavelength would be the best one to use to measure the concentration of protein in a solution? Why this wavelength and not another wavelength 100nm higher or lower? 5. Below is the absorption spectra for Coomassie Blue - the dye involved in the Bradford Assay. What are the concentrations of the 2 unknown hemoglobin samples that you measured in lab? Use (and show your work) the regression line equation to calculate the concentration of the unknown solution. What do the different values in the equation represent? 3. Record the equation for the regression line. Description - see: Writing Papers in the Biological Sciences for good examples of legend writing. Paste in a labeled copy of your graph as a figure here in your answer and write an appropriate descriptive legend below it (Figure 1). Then insert a trend line (linear regression line) to form the standard curve. ![]() Use Logger Pro to generate a standard curve for the BSA standards that you measured in the spectrophotometer.
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12/29/2023 0 Comments Hdd molex connectorThis cable also demonstrates the ability to detach the last 4 pins. To create backwards compatibility most modern power supplies allow you to disconnect the last 4 pins of the main connector. It is also possible to create forward compatibility by using an adapter. In total there are ober 12 versions of the ATX standard, but they are so similar that you do not need to worry about compatibility With the introduction of ATX12 V2.0 are change was made to a system with a 24-pins connector. If you don’t 75W will flow through your Molex cable. This reduces the risk of overloading your power supply. ![]() Please make sure you connect both molex to different cable strains. Need another PCI-6 pin cable to power your graphics card? Use the “2x Molex to 1x PCI-E 6-pin” adapter. Old power supply unit or simply lacking the required number of SATA power connectors? Use a Molex to SATA connector to power your latest hard disk drive. Basically they were superseded by the USB stick. For those of you who do not remember these were square magnetic disks that could contain up to 1.44 MB of data. These guys were used to power floppy disk drives. Mini-Molex / Floppy connectorĬompletely obsolete, but some PSU’s still come with a mini-molex connector. Thanks to their L-shape the SATA power connector can only connected the right way. All modern DVD-players, hard disk drives and SSD’s are powered by SATA power. The SATA connector is the guy that made the Molex obsolete. Keep in mind that they can be extremely difficult to detach. Thanks to its angular side you cannot go wrong when connecting a Molex cable. However their power draw is limited so nowadays most of their purpose has been replaced by PCI-E cables and SATA cables. Even some graphics cards like the Geforce 7800 GS were equipped with Molex. In the past these guys were often used to connect Hard drives, CD-ROM players, etc. Molex connectors have been around for a very long time and can deliver 5V (red) or 12V (Yellow) to hardware peripherals. With it’s 8 pins this connector can provide up to 150W per cable. A graphics card with a single 6+2-pin connector can draw up to 225W (75W from the motherboard + 150W from the cable). More expensive graphic cards require the 6+2 pin PCI-E connector. So if your Graphic card contains a single 6-pin connectors it can draw up to 150W (75W from the motherboard + 75W from the cable). The PCI-E 6-pin connector can supply an additional 75W per cable. The left 2 pins of the 6+2 pin connector on the right is detached to provide backwards compatibility with 6-pin graphic cards. To solve that issue the PCI-E connector was introduced. Faster dedicated graphics cards require much more power. The motherboard can provide a maximum of 75W through its PCI-E interface slot. It is also possible that your 8-pin cable can be split into two segments to provide backwards compatibility with cheaper motherboards. Obviously you only need to use one of these cables. Most PSU’s provide two cables one with 4-pins and one with 8-pins. For regular usage there is absolutely no need for the additional pins. The extra 4 pins ensure that enough power can be provided to the cpu when overclocking. More expensive “overclocking” motherboards have 8-pin connectors. Nowadays it is the P4, or EPS connector, to provide the cpu with power.Ĭheap motherboards are equipped with a 4-pins connector. With overclocked cpu’s drawing as much as 200W a need to provide power directly to the CPU was created. P4 (EPS Connector)Īt some point in time the motherboard’s pins were no longer sufficient to provide the processor (cpu) with power. ![]() ![]() A 24-pins cable is backwards compatible with a 20-pins motherboard, often this cable can be split into 20- and 4-pins (like in the image above). 12/29/2023 0 Comments Smart battery isolator![]() The isolator kit will begin charging the. The Smart Start SBI12 battery isolator is a safe way to charge a dual battery system. This dual battery isolator kit allows you to charge two batteries at the same time from your engine alternator. It features a low standby current draw and sophisticated fault detection and LED indication of operation to warn the user of faults including over-voltage, voltage drop and excessive current draw conditions. Allows bi-directional charging from the alternator or from shore power. There are no efficiency losses due to diodes. Smaller and lighter than traditional isolators. Simple to install - connect to starting battery, auxiliary battery and ground. It is better than a diode battery isolator due to the voltage drop associated with diode type isolation. More flexible than traditional battery isolators. The battery isolator is primarily used in 4WD and commercial vehicles and incorporates electronic components that will prevent the solenoid from generating high voltage transients. The SBI2 provides 12 volt DC incorporating 100A of continuous ratings. It will protect the start battery from excessive discharge, whilst allowing the auxiliary battery to supply non-essential loads. The REDARC Smart Start® SBI2 is a microprocessor-controlled battery isolator used in dual battery systems as a solenoid priority system. Great Quality at Low Prices lowest prices around 250A 12V-24V Automotive Smart Dual Battery Isolator Power Batteries Split Relay Lifestyle shop for people. It comes with a sensitive chip that ensures the primary battery is. This is an ideal battery isolator for charging a dual battery system. It is a top-notch quality system with rugged durability. Smart Start SBI 12V 100A Redarc B attery Isolator. Cons: Cables could be a little longer Check Price on Amazon. ![]() Note, turning on Wi-Fi for a few minutes has little or no impact on your battery life. Hey guys, Just wondered if anyone uses the Snapdragon Battery Guru app I used to with my nexus 4 and 5 and I thought it helped, but not too sure if it's helped much with my G3 Been getting anywhere from 3-4.5 hours SOT. Likewise when you move to a new location, BatteryGuru will turn Wi-Fi on for a few minutes to check if Wi-Fi access is available. However, even when you don’t have Wi-Fi access Snapdragon BatteryGuru will periodically turn Wi-Fi on for few minutes to check if your ability to access Wi-Fi has changed. If you have a compatible device then the Snapdragon Battery Guru comes highly. ![]() There will be one activated setting under Battery Saver called Battery. After the initial learning period completed, I noticed around a 30 increase in battery life with no noticeable change in app performance. The LG G4 comes with a powerful Qualcomm MSM8992 Snapdragon 808 chipset which is. After Qualcomm released Battery Guru (Beta) application for Android which optimizes Snapdragon-powered devices to offer more battery life. Snapdragon BatteryGuru’s Wi-Fi management feature turns Wi-Fi on when you need it and turns it off when you don’t, thereby saving you power. The app can take control of Bluetooth and also Wi-Fi, switching it on when you’re in range of a router and off when you leave. You can also choose to turn on the battery saver tool, which crimps. Why is my Wi-Fi on sometimes, when I’m expecting it to be off?A. Googles latest mobile operating system, Android 6.0 Marshmallow, is a tasty treat. It does not use GPS, but instead uses Android’s low power network location mechanism to find your approximate location. Snapdragon BatteryGuru uses your location to know the places where you have access to Wi-Fi. Why does Snapdragon BatteryGuru need to know my location?A. If you experience problems using this battery saver app, please send us an email at explaining your problem prior to leaving a review – we’ll try to respond as soon as possible. Please note, BatteryGuru does not currently support Android M. Processor, 1.4GHz Qualcomm Snapdragon octa core processor deliver ldelivers smooth. ![]() Snapdragon BatteryGuru automatically learns and adjusts the smartphone settings so you don’t have toĪfter a 7 day initial learning period, Snapdragon BatteryGuru learns the user’s behaviors and continues to operate in the background, deepening its understanding of the user and further optimizing battery life over time. carrier models have the Exynos 8890 chipset.
![]() Click "Insert Field" to see a list of available macros. ![]() "%artist%") are macros which will be replaced by the corresponding tag data for each file. Here you can create your own naming format. If you don't like any of the preset styles, choose "Create custom entry" to create your own naming format in the next step. Please select your preferred file naming style. In each entry in the list below, the first line is an example of how a release with a single artist would look, and the second line is how a release with "Various Artists" would look. Picard can use different naming styles, depending on whether the release is a "Various Artists" release, or a single artist. How would you like your files to be named? Question: do you mean "delete empty files", or maybe "delete empty directories" ? Answer: Exactly :) - Naming Format NB operations take effect only when Picard saves a file. *.jpg for cover art image files and *.txt for lyrics text files. Picard can be directed use file type to identify non-audio files Į.g. To move the other files use the "Move additional files" option.For example, to categorize by "artist name", enter the name of the top level music folder. To organize your files into sub-folders.To move your music files to another folder, use the "Move files" option, and enter the name of the target folder.On the following screens you'll be asked how you'd like the files to be renamed. To rename your music files based on the tag data, use the "Rename files" option.Picard can tag and organize your music files according to your preferences. This wizard will help you configure Picard to organize your music the way you want it, and it will introduce you to the enhanced tagging features of the Picard program. ![]() Welcome to the Picard Music File Tag Manager configuration wizard! 12/29/2023 0 Comments Dmc 5 microtransactionsIf a player instantly unlocks all upgrades from the get-go, sure they’ll have a lot of options but they might also find themselves overwhelmed, as opposed to a player who slowly unlocked upgrades over time and gained the requisite mastery along the way. Throughout the entire DmC series, more complex upgrades and character maneuvers have been purposefully made more expensive (in terms of Red Orb cost) since they require more time and skill to master. Itsuno went on to explain that, while instantly unlocking all upgrades via Red Orb microtransactions is technically possible, it may in fact not be the best strategy for players who want to truly master the game’s combat tactics. You should be able to play it the way you want to play it.” But on the other hand I don’t feel you have to get all the moves. ![]() ![]() Devil May Cry 5 launches on PC, PlayStation 4 and Xbox One on March 8th. If they want to save time and just want to get all the stuff at once, those people can do that. According to Capcom's producer Matt Walker, Devil May Cry 5 microtransactions will not affect balance at all. So you have to make the decision between going for the cheaper stuff or saving up, getting the thing that has a lot of application but you’ll have to spend time learning and perfecting.ĭevil May Cry is set to release sometime in Spring 2019.“With giving people the ability to purchase Red Orbs, it’s something we want to give people as an option. Partly because of you save up for that you’re not going to be able to buy as many skills, so you’re going to have the time to learn it. So people will think, ‘Oh this is cheap so I’m just going to buy this.’ But then for the stuff that’s going to be harder to use and master, we make that more expensive. (The US average is 22.7) Fawn Creek property crime is 18.4. Fans have been able to play DMC 5 at TGS 2018 this week. The first thing is the stuff that we feel people should want to get first is made cheaper. Crime is ranked on a scale of 1 (low crime) to 100 (high crime) Fawn Creek violent crime is 9.6. A TGS demo and PlayStation Store listing have revealed Devil May Cry 5 will have both microtransactions and some form of online multiplayer. It’s interesting because from a game design point of view there’s two different things we think about when we set the prices of the moves, skills, and abilities. Peter Brown also asked how the microtransactions would impact the learning/progression cycle of playing Devil May Cry 5. Microtransactions for increased character strength also existed in Devil May Cry 4 Special Edition. You should be able to play it the way you want to play it. If they want to save time and just want to get all the stuff at once, those people can do that. With giving people the ability to purchase Red Orbs, it’s something we want to give people as an option. He answered that it’s about giving players the option to play as they wish: Just confirmed that the games microtransactions wont be just cosmetic but will give people the option to buy red orbs, resulting in people just Press J to jump to the feed. GameSpot writer Peter Brown spoke to Hideaki Itsuno, the director of Devil May Cry 5, about why they decided to put a microtransaction system into the game, which is one with no sort of competitive multiplayer. It’s a progression cycle that encourages players to hone their skills to make their characters stronger. The orbs can be spent to improve your character in various ways, including unlocking newer, deadlier combos. ![]() ![]() Doing better in combat and perfecting combos cause the enemies to drop more orbs. In the Devil May Cry games, a big part of fighting is performing combos on enemies to earn orbs and higher battle ranks. 12/29/2023 0 Comments Cadillacs and dinosaurs pc![]() ![]() Download cadillacs and dinosaurs.cadillacs and dinosaurs game free download cadillacs and dinosaurs information cadillacs and dinosaurs game trailer cadillacs and dinosaurs system.cadillacs and dinosaurs pc game free download cadillacs and.download cadillacs and dinosaurs game from high speed direct download li.amazon: cadillacs and.Įnter your username to access personal online banking.cadillacs and dinosaurs pc game file size:12.9mb system requirements cpu: 233mhz ram: 64mb os: windows 98, 2000, xp, vista, 7 and windows 8 game play instructions.cadillacs and dinosaurs game information game name cadillacs and.play cadillacs and dinosaurs capcom cps 1 game online for free in your browser.cadillacs and dinosaurs pc game free download cadillacs and dinosaurs game for. Free.the xenozoic age q world 0 years in the future, where dinosaurs of the past co exist.ĭinosaurs.cadillacs and dinosaurs download best and latest game for play on your pc this is a best game to play with your friends. Pc.if you enter it differently from the.remember, your username is case sensitive.cadillacs and dinosaurs game information game name cadillacs and dinosaurs developers capcom publishers capcom platforms microsoft windows, arcade release date.direct download via magnet link.cadillacs and dinosaurs pc full. 12/29/2023 0 Comments Lightspeed stock priceI added 3K shares thus morning as the possibility if an earnings and revenue surprise increased this morning with Shopify's decent quarterly results. how much will Lightspeed's stock appreciate? considering how far it has come off its 52 week highs, any favourable Q3 revenue and earnings result could proof to be very, very nice to Lightspeed's share price. If Lightspeed beats the lowered Q3 forecasts, could we see a nice bump up in Lightspeed's stock price? Shopify has been up close to 15% all morning today as a result of their beat. Shopify's Q3 results were better than forecasted and the market LOVES IT. Market Cap 2,733.25M Shares Out 149.37M 10 Day Average Volume 1.18M Dividend - Dividend Yield - Beta 2.99 YTD Change -53.29 RATIOS/PROFITABILITY EPS (TTM) -2.98 P/E (TTM) -8.00 Fwd P/E (NTM) -. Get the latest Lightspeed Commerce Inc (LTS:0A8V) stock price, GURU trades, performance, financial stability, valuations, and filing info from GuruFocus. The thinking was revenues had a chance to be strong once again this year and therefore were not factored into the recent analyst forecasts for Shopify and Lightspeed's Q3 results. What will drive Lightspeed stock price in 2021 To understand the catapult effect, let’s go back to September 2020, when Lightspeed started trading on the New York Stock Exchange, raising US397.7 million in an initial public offering (IPO). According to 12 Wall Street analyst s that have issued a 1 year LSPD price target, the average LSPD price target is 48.25, with the highest LSPD stock price forecast at 103. Recently, there was indications Q3 results for both companies could beat lowered expectations. The pullback acts like a catapult and drives the stock price momentum. ![]() Lightspeed currently has 700 employees spread across eight locations in Canada, the U.S., Europe and Australia, and has customers in 100 countries around the world.Nice quarterly beat by Shopify could be a very nice pre- cursor to Lightspeed's upcoming reported results. Do you own or manage a golf course Provide an exceptional golfing experience through industry-leading technology. "We are very proud to welcome a leading-edge technology company and homegrown Canadian innovation story to our market," TSX's president of capital formation, Loui Anastasopoulos, said in a release.ĭespite going public, the company's dual-share voting structure means that controlling interest in the company will remain with company insiders, including CEO and founder Dax Dasilva. raised about half that much in its 2015 IPO, and that company has since grown to be worth $27 billion. That officially makes the company what's known as a unicorn in start-up circles - a valuation of $1 billion before going public.Īt $240 million, the launch officially makes Lightspeed one of the 10 biggest technology IPOs in the history of the Toronto Stock Exchange. The IPO has sold off just under one fifth of the company for $240 million, giving the company a total value of $1.4 billion. On average, they anticipate the companys stock price to reach C59.29 in the next year. That's because the offering was more popular than anticipated, so they were able to raise their price. Their LSPD share price forecasts range from C30.00 to C128.00. The $16 IPO price is higher than the range of $13 to $15 the company had given as an expected range when it announced its plans to go public last month. ![]() The shares were trading at just over $20 each near midday, with more than 5 million shares changing hands, and closing at $18.90. With analysts defining 20.91-65.00 as the low and high price targets, we arrive at a consensus price target of 36.07 for the trailing 12-month period. Friday is the first day the general public can buy the shares on the stock market, and they are eagerly bidding them up in early trading. Is Lightspeed Commerce stock a buy or sell 100 free analysis on LSPD, no signups required. (NYSE:LSPD)’s beta value is currently sitting at 2.96, while the Average True Range indicator is currently displaying 1.37. It raised $240 million in an initial public offering on Thursday, selling 15 million shares for $16 each. The one-stop commerce platform for retail, hospitality and golf merchants around the world to simplify, scale and provide exceptional customer experiences. NuORDER is changing how brands and retailers collaborate to shop in-season and upcoming collections, access real-time product data and visually plan. The stock traded as low as 17.82 and last traded at 17.88.
12/29/2023 0 Comments Free wedding salon 2 game![]() There are several time management titles available in a single pack, so the player needs to play all titles one by one to have fun. During the gameplay, the player will reveal the uplifting the storyline and head to the diner kitchen to cook yummy meal while matching and serving his way to sweet success. Big ad his minions as they cook up their plans. In the game, she has to fix the town his way and struggle to fend off an evil guy named Mr. While playing the game, the player has to reveal the breathtaking storyline of the heroine named Flo, who returns to her homeland after a decade to help the citizens and animals of DinerTown. The story centers on a closed hotel, in which the diner is shambles and the kitchens are no longer available to cook yummy food. There are several characters to play with, and each has unique personality and abilities. The game places the player on an epic adventure where the player joins a female protagonist to restore a restaurant to its glory. ![]() Wedding Salon 2 includes core features such as different Locations, 154 Levels, Mini-Games, and more.ĭiner DASH Adventures by Glu is an Adventure, Time Management, and Single-player Strategy video game released for mobile devices. There are over 154 tricky levels available and each incorporates with time management genre. During the gameplay, the player can make a weeding boutique chain to become the true master of wedding preparations, also baking a wedding cake, design clothes, and more. It introduces a cheerful and entertaining success storyline with several mini-games. The long-awaited sequel appears with lots of new content and challenging objectives to complete. While playing the game, the player must open and create a chain of wedding salons for shopping all across the globe, including Bali, Italy, and France, etc. In the game, the player must hire staff and broaden the range of fashion products to become the celebrity. The game was developed by Sugar Games and set in your dream worlds where you as the main character must create and manage your salon for free. ![]() Wedding Salon 2 is a Time Management and Single-player Simulation, serving as the sequel to Wedding Salon for Android and iOS. 12/28/2023 0 Comments Tekken 3 fight games![]() It has sold 9 million copies because it's so fun and addictive and it broke the mold for fighting games to come. This game is still being bought to this day. The only flaw the game had was the poor bonus characters (Dr Boskonvitch and Gon) with their awful movesets and the lackluster mini game modes. It also gave us Tekken Force and Tekken Ball mode. It introduced the evade/sidestep, the left/right side body throws and ukemi mechanics. ![]() The gameplay was more refined in terms of juggling, combos and damage balancing. The new characters to the game and series were pretty cool. The sound effects are and still so badass like the battle cries, punches, kicks and deathcries. The console version soundtrack is amazing but nowhere near iconic as the arcade version. They would become much smoother, stylized, crisp and realistic as the series would continue on. The graphics were a huge improvement over the polygon, block look of the previous games. So many childhood memories from this game growing up. 1998, one of the best years in video game history period. They would become much smoother, stylized, crisp and The game that REVOLUTIONIZED the fighting game genre when the Street Fighter series was starting to decline. The game that REVOLUTIONIZED the fighting game genre when the Street Fighter series was starting to decline. This has got to be one of the best fighters that have ever existed, topped only by Tekken 5 or Soul Calibur (also by Namco). My personal favourite is Lei Wulong, for all the 5 forms he has. There isn't really a best character, because Every character can defeat and be defeated by any other, again, why I favour this game. ![]() By playing with your friend, you'll probably learn a new move, unless you've already mastered all of the 100+ moves your character has. You can't really spam one move and win (which is why I find it hundreds of times better than other fighter games), there's a lot of combo potential. ![]() The models look great, especially for a PS1 game, the environments are rich and diverse. The cutscenes were an unforgettable touch. There are many game modes, all equal in the fun they provide. I find this game much more suited for multiplayer, butthe single player game is great, the bots are fair, and there's a ton to unlock. Whenever me and my best buddy are feeling competitive, we turn on the good old PS2, and play Tekken 3 for hours, constantly filled with adrenaline. |
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